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Journal: Frontiers in Drug Delivery
Article Title: Single cell phototransfection of mRNAs encoding SARS-CoV2 spike and nucleocapsid into human astrocytes results in RNA dependent translation interference
doi: 10.3389/fddev.2024.1359700
Figure Lengend Snippet: Phototransfection Process and Visualization in Rat PC12 Cells – (A) Schematic representation of the phototransfection set-up. (B) An intact PC12 cell is circled and appears black entity (lacking fluorescence) as it is surrounded by fluorescein which is excluded from the cell. (C) Schematic illustration demonstrating the phototransfection irradiation process using multiple laser light pulses in a 3 × 3 pattern over 1,260 nm 2 of the cell membrane. This process introduces transient holes into the cell membrane. (D) Following laser irradiation, fluorescein diffuses into the cell through the holes created in the cell membrane. The PC12 cell now appears white due to fluorescence of the internalized fluorescein. Scale bar is 45 microns.
Article Snippet: The
Techniques: Fluorescence, Irradiation, Membrane
Journal: Frontiers in Drug Delivery
Article Title: Single cell phototransfection of mRNAs encoding SARS-CoV2 spike and nucleocapsid into human astrocytes results in RNA dependent translation interference
doi: 10.3389/fddev.2024.1359700
Figure Lengend Snippet: Phototransfection of eGFP mRNA into Rat PC12 Cells, Human Astrocytes and Mouse 3T3 cells – (A) A phototransfected PC12 cell appears green from expression of eGFP protein after phototransfection with eGFP-encoding mRNA (0.38 μg/mL in the PBS surrounding the cell). Inset shows lack of fluorescence in nontransfected PC12 cells. (B) Two human astrocytes exhibit eGFP protein fluorescence after phototransfection with eGFP mRNA (0.38 μg/mL). Inset shows lack of fluorescence signal in nontransfected human astrocyte cells. (C) Mouse 3T3 cells also express eGFP after phototransfection (0.38 μg/mL). (D) Quantification of eGFP fluorescence data from multiple cell phototransfections. PC12 cells (N = 39) exhibited higher eGFP expression compared to human astrocytes (N = 59) and mouse 3T3 cells (N = 43). Data are presented as mean ± SEM. ***, p < 0.001. Scale bar is 45 microns.
Article Snippet: The
Techniques: Expressing, Fluorescence